eDNA metabarcoding evaluated for fish diversity assessment

Environmental DNA (eDNA) metabarcoding has emerged as a powerful tool for monitoring aquatic biodiversity, enabling researchers to identify fish species from traces of DNA found in water without using invasive techniques on fish. Although this technique is gaining traction, it introduces certain biases and uncertainties at various stages, thereby affecting the production of reliable eDNA results. This challenge underscores the need to understand the technique's strengths and limitations.